|
KMID : 1025520000420020165
|
|
Journal of Animal Science and Technology
2000 Volume.42 No. 2 p.165 ~ p.172
|
|
|
Retinoic Acid Levels and Function of Porcine Thyroid Cells In Vitro
|
|
|
|
|
Abstract
|
|
|
|
The effect of retinoic acid (RA) on iodide metabolism in the thyroid was studied in an in vitro culture model. Porcine thyroid follicular cells were incubated with Coon´s modified Ham´s F-12 medium with or without RA (10^(-8)-10^(-5)M) for 48 hours, and cell proliferation, iodide uptake, iodide organification and intracellular cAMP concentration were determined. RA did not influence the proliferation of thyroid cells. However, marked inhibition of the iodide uptake was observed when incubated with 10^(-8), 10^(-7), 10^(-6) and 10^(-5) M RA as compared with control (p $lt; 0.05). Iodide organification reflecting thyroid hormone synthesis was also inhibited by addition of RA (p $lt; 0.05). Pretreatment of thyroid cells with RA caused a significant inhibition of TSH-stimulated cAMP accumulation (p $lt; 0.05) in the cells. The results obtained indicated that RA inhibited iodine metabolism by reducing TSH-stimulated cAMP response and also by acting on the metabolism steps subsequent to cAMP production in thyroid cells.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|